transitlab

06 Feb, 2010

Kiwi Fruit Dna Isolation 30/365

Posted by: Brian In: workshop

Image by sctv via Flickr

For the ptechnic lab (http://ptechnic.org/ecologies/b-ecologies/index.html), I demonstrated DNA extraction from kiwifruit, they have buckets of DNA.

Simple recipe, mash up kiwifruit in a 100ml of detergent/salsolution ( 70ml detergent 90gm salt in a l of water). Then heat gently for 15 min, strain and gently add some isopropanol. DNA will start to precipitate at the interface between the phases. Its bucket chemistry. Its lovely!

pdf version: Isopropanol based isolation of dna from kiwifruit (bioartlab)

Recipe based on www.york.ac.uk/res/sots/activities/diydna.htm but using isopropanol instead of methylated spirits.

Tags: dna, isolation, kiwifruit

1 Response to "Kiwi Fruit Dna Isolation 30/365"

1 | Mac Cowell

March 1st, 2010 at 11:02 pm

If you enjoy this little DNA exercise, you might like my related DNA in a Shot Glass:

http://www.instructables.com/id/5_minute_DNA_Extraction_in_a_Shot_Glass/

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About

Brian Degger is a technologist/artist, he writes, thinks and makes around themes of interactivity, biomimicracy, and collaboration

Asides

PDF import and editing in openoffice
One function I have found useful in openoffice is the ability to import pdfs. This is possible by downloading and installing the pdfimport plugin. This means that you can import a pdf, edit it then output it at a lower resolution for screen. (0)

miniFM

In the foreground is a Tetsuo Kogawa version (built during RadioCraftLab, during AVFest08) and in the back the remix by sonodrome (built last week at Sonodrome Central). Both assembled by me.

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Bioplastic
Jay Cousins and friends had a hackday around bioplastics and laser cutting. Making the bioplastic from starch and glycerin (see this link for more details) and then laser cutting them. Cool stuff, they were even making different color ones. This is important as people move from using commercially sourced plastic in their makerbots to something else. Plastic is expensive to buy for these machines, but it is all around. SO be it bio-plastic or post waste plastic, ways of reusing these provide a compelling reason to throw away less waste. It is amazing that these materials are coming out of the factories, to be used in domestic situations. Together with polymorph and sugru, there are a variety of materials to play around for wearables, for prototypes and one offs. Bring on the future, with peer production.

Maker Faire Newcastle is March 13-14, 2010 (makezine.com)
Bioplastics – the quest for Open Source material production (jaycousins.wordpress.com)

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augmented foraging - cool use of layar -

augmented foraging Originally uploaded by _foam A mobile phone guide to edible urban wild-food sources.

Amsterdam urban_ edibles is developing Augmented_Foraging, a mobile phone guide to wild-food sources using Layar. Much better use of this program than finding property in Amsterdam (unless you live there of course)!

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Kiwi Fruit Dna Isolation 30/365